Composite

Part:BBa_K3338012:Design

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)


CMV-EGFP-MagA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2553
    Illegal BsaI.rc site found at 1885
    Illegal BsaI.rc site found at 2434
    Illegal SapI site found at 1575


Design Notes

MagA has to be in frame with EGFP.


Source

CMV and EGFP from pEGFP-C2, MagA from Magnetospirillum magneticum

References